Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 fromHuman coronavirus HKU1
نویسندگان
چکیده
منابع مشابه
Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.
Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. T...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications
سال: 2014
ISSN: 2053-230X
DOI: 10.1107/s2053230x14023085